| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2567-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99418-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17687-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2567 | |||
| Carrier Free | AF2567-SP | |||
![Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SHP-77 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiASCL1/MASH1 recombinant monoclonal antibody used for IP at 6 ul per reaction.ASCL1/MASH1 was also immunoprecipitated by a secondantibody against a different epitope of ASCL1/MASH1 For blotting immunoprecipitatedASCL1/MASH1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-NBP3-14738-img0001.jpg "Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SHP-77 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiASCL1/MASH1 recombinant monoclonal antibody used for IP at 6 ul per reaction.ASCL1/MASH1 was also immunoprecipitated by a secondantibody against a different epitope of ASCL1/MASH1 For blotting immunoprecipitatedASCL1/MASH1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin:ASCL1/Mash1 Antibody [NBP3-14738] -ASCL1/MASH1 recombinant monoclonal antibody [BLR164J]. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14738_rabbit-ascl1-mash1-mab-immunohistochemistry-paraffin-26720247516..jpg)
![Western Blot: ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (50 ug) from HEK293T, HeLa, SHP-77, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/nbp3-14738_rabbit-ascl1-mash1-mab-western-blot-267202475335..jpg)
![Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody (2D9) [H00000429-M02] - Analysis of monoclonal antibody to ASCL1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-2D9-Immunocytochemistry-Immunofluorescence-H00000429-M02-img0002.jpg "Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody (2D9) [H00000429-M02] - Analysis of monoclonal antibody to ASCL1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159] - ASCL1/Mash1 antibody detects ASCL1/Mash1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASCL1/Mash1 stained by ASCL1/Mash1 antibody (NBP3-13159) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13159-img0002.jpg "Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159] - ASCL1/Mash1 antibody detects ASCL1/Mash1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASCL1/Mash1 stained by ASCL1/Mash1 antibody (NBP3-13159) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: ASCL1/Mash1 Antibody [NBP3-13159] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1/Mash1 antibody (NBP3-13159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13159_rabbit-ascl1-mash1-pab-9102024201572.jpg)
![Western Blot: ASCL1/Mash1 Antibody [NBP2-99418] - Anti-ASCL1/Mash1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 28 kDa](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-Western-Blot-NBP2-99418-img0006.jpg "Western Blot: ASCL1/Mash1 Antibody [NBP2-99418] - Anti-ASCL1/Mash1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 28 kDa")
![Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418] - ASCL1/Mash1 was immunoprecipitated using: Lane A: 0.5 mg Mouse brains Tissue Lysate 4 ul anti-ASCL1/Mash1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ASCL1/Mash1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-Immunoprecipitation-NBP2-99418-img0004.jpg)
![SDS-Page: Recombinant Human ASCL1/Mash1 Protein [H00000429-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ASCL1-Mash1-Protein-SDS-Page-H00000429-Q01-img0001.jpg "SDS-Page: Recombinant Human ASCL1/Mash1 Protein [H00000429-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: ASCL1/Mash1 Antibody (1C5) [H00000429-M04] - Analysis of ASCL1 expression in A-549 (Cat # L025V1).](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-1C5-Western-Blot-H00000429-M04-img0005.jpg "Western Blot: ASCL1/Mash1 Antibody (1C5) [H00000429-M04] - Analysis of ASCL1 expression in A-549 (Cat # L025V1).")
![ELISA: ASCL1/Mash1 Antibody (1C5) [H00000429-M04] - Detection limit for recombinant GST tagged ASCL1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-1C5-ELISA-H00000429-M04-img0003.jpg)
![Western Blot: ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - Analysis of ASCL1 expression in transfected 293T cell line by ASCL1 monoclonal antibody (M01), clone 7E11.Lane 1: ASCL1 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-7E11-Western-Blot-H00000429-M01-img0004.jpg "Western Blot: ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - Analysis of ASCL1 expression in transfected 293T cell line by ASCL1 monoclonal antibody (M01), clone 7E11.Lane 1: ASCL1 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.")
![ELISA: ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - Detection limit for recombinant GST tagged ASCL1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-7E11-ELISA-H00000429-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-17687] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ASCL1-Mash1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17687-img0001.jpg "Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-17687] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.")
![Western Blot: ASCL1/Mash1 Overexpression Lysate [NBL1-07759] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASCL1/Mash1.](http://images.novusbio.com/fullsize/MASH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07759-img0002.jpg "Western Blot: ASCL1/Mash1 Overexpression Lysate [NBL1-07759] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASCL1/Mash1.")