ASC/TMS1 Antibody

Images

 
Western Blot: ASC/TMS1 Antibody [NB100-870] - staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NB100-870] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NB100-870] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ASC/TMS1 Antibody Summary

Immunogen
Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2; NP_660183.1.
Epitope
C-RESQSYLVEDLERS
Localization
Cell Membrane. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appeared as hollow, perinuclear spherical, ball-like structures.
Specificity
This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx. 24kDa band observed in lysates of cell line U937 (calculated MW of 21.6kDa according to NP_037390.2).
IHC: In paraffin embedded Human Liver shows staining of sinusoidal lining cells.
Positive Control
ASC/TMS1 Lysate (NBL1-15016)
Publications
Read Publication using NB100-870.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

  • ASC
  • ASCMGC10332
  • CARD5
  • CARD5TMS
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain containing
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS1
  • TMS-1
  • TMS1apoptosis-associated speck-like protein containing a CARD

Background

ASC (apoptosis-associated speck-like protein containing a CARD) or TMS 1 (target of methylation-induced silencing) is a recently identified CpG island-associated gene that becomes hypermethylated and silenced in cells overexpressing DNA cytosine-5-methyltransferase-1. TMS1 is aberrantly methylated and silenced in human breast cancer cells. Forty percent (11 of 27) of primary breast tumors exhibited aberrant methylation of ASC. Ectopic expression of ASC induced apoptosis in 293 cells and inhibited the survival of human breast cancer cells. The methylation-mediated silencing of ASC may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. ASC encodes a 22-kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of ASC alone was able to trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball-like structures. Several lines of evidence support the idea that redistribution of ASC is an intermediate step in a ASC-triggered apoptotic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASC/TMS1 Antibody (NB100-870)(1)

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WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on ASC/TMS1.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Bioinformatics

Gene Symbol PYCARD
Entrez
Uniprot