Arylsulfatase A/ARSA Antibody

Arylsulfatase A/ARSA was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Human Arylsulfatase A/ARSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2485) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Arylsulfatase A/ARSA Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ARSA
  Arg19-Ala507
  Accession # AAH14210
• Specificity: Detects human ARSA in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse ARSA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARSA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arylsulfatase A/ARSA Antibody

• ARSA
• Arylsulfatase A
• ASA
• Cerebroside 3-sulfatase
• Cerebroside-sulfatase
• EC 3.1.6
• EC 3.1.6.8
• MLD

Background

As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Recombinant human ARSA corresponds to the mature chain and has sulfatase activity described above.

1. Lukatela, G. et al. (1998) Biochemistry 37:3654.
2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
3. Stein, C. et al. (1989) J. Biol. Chem. 264:1252.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arylsulfatase A/ARSA Antibody (AF2485)(1)

Publication using AF2485

• Yamazaki Y, Hirai Y, Miyake K, Shimada T Targeted gene transfer into ependymal cells through intraventricular injection of AAV1 vector and long-term enzyme replacement via the CSF. Sci Rep, 2014;4(0):5506. 2014 [PMID: 24981028] (IHC - Frozen,, Mouse)

Bioinformatics

• Gene Symbol: ARSA
• Entrez:
  • 410(Human)
  • 11883(Mouse)
• Uniprot:
  • AAH14210()