Arylsulfatase A/ARSA Antibody [Unconjugated]

Arylsulfatase A/ARSA was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Human Arylsulfatase A/ARSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2485) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Arylsulfatase A/ARSA Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ARSA
  Arg19-Ala507
  Accession # AAH14210
• Specificity: Detects human ARSA in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant mouse ARSA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARSA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arylsulfatase A/ARSA Antibody [Unconjugated]

• ARSA
• Arylsulfatase A
• ASA
• Cerebroside 3-sulfatase
• Cerebroside-sulfatase
• EC 3.1.6
• EC 3.1.6.8
• MLD

Background

As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Recombinant human ARSA corresponds to the mature chain and has sulfatase activity described above.

1. Lukatela, G. et al. (1998) Biochemistry 37:3654.
2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
3. Stein, C. et al. (1989) J. Biol. Chem. 264:1252.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arylsulfatase A/ARSA Antibody (AF2485)(3)

Publications using AF2485

• Xinying Hong, Arun Babu Kumar, Jessica Daiker, Fan Yi, Martin Sadilek, Fabiola De Mattia et al. Leukocyte and Dried Blood Spot Arylsulfatase A Assay by Tandem Mass Spectrometry Analytical Chemistry 2020-05-05 [PMID: 31922725]
• Christine í Dali, Norman W. Barton, Mohamed H. Farah, Mihai Moldovan, Jan‐Eric Månsson, Nitin Nair et al. Sulfatide levels correlate with severity of neuropathy in metachromatic leukodystrophy Annals of Clinical and Translational Neurology 2015-05-01 [PMID: 26000324] (ELISA Capture, Human)
• Yamazaki Y, Hirai Y, Miyake K, Shimada T Targeted gene transfer into ependymal cells through intraventricular injection of AAV1 vector and long-term enzyme replacement via the CSF. Sci Rep, 2014-07-01;4(0):5506. 2014-07-01 [PMID: 24981028] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: ARSA
• Uniprot:
  • AAH14210()