ARX Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

ARX Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human ARX
  Ser2-Ala100
  Accession # Q96QS3
• Specificity: Detects human ARX in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARX Antibody [Alexa Fluor® 488]

• aristaless related homeobox
• Aristaless-related homeobox
• aristaless-related homeobox, X-linked
• ARX
• cancer/testis antigen 121
• CT121
• EIEE1
• homeobox protein ARX
• ISSX
• ISSXmental retardation, X-linked 36
• mental retardation, X-linked 29
• mental retardation, X-linked 32
• mental retardation, X-linked 33
• mental retardation, X-linked 38
• mental retardation, X-linked 43
• mental retardation, X-linked 54
• mental retardation, X-linked 76
• mental retardation, X-linked 87
• MRX29
• MRX32
• MRX33
• MRX36
• MRX38
• MRX43
• MRX54
• MRX76
• MRX87
• MRXS1
• PRTS

Background

ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27-34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328-387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96QS3(Human)