ARPC2 Antibody (4F2G6)

Western Blot: ARPC2 Antibody (4F2G6) [NBP3-15471] - Western blot analysis of extracts of various cell lines, using ARPC2 antibody (NBP3-15471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: ARPC2 Antibody (4F2G6) [NBP3-15471] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug ARPC2 antibody (NBP3-15471). Western blot was performed from the immunoprecipitate using ARPC2 antibody (NBP3-15471) at a dilition of 1:1000.

Immunocytochemistry/ Immunofluorescence: ARPC2 Antibody (4F2G6) [NBP3-15471] - Immunofluorescence analysis of NIH/3T3 cells using ARPC2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: ARPC2 Antibody (4F2G6) [NBP3-15471] - Immunofluorescence analysis of PC-12 cells using ARPC2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clone: 4F2G6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ARPC2 Antibody (4F2G6) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ARPC2 (O15144). FSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARPC2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ARPC2 Antibody (4F2G6)

• actin related protein 2/3 complex, subunit 2 (34 kD)
• actin related protein 2/3 complex, subunit 2, 34kDa
• ARC34actin-related protein 2/3 complex subunit 2
• Arp2/3 complex 34 kDa subunit
• ARP2/3 protein complex subunit 34
• p34-Arc
• PNAS-139

Background

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARPC2