Aromatase Antibody

Western Blot: Aromatase Antibody [NBP2-80415] - Analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-80415] - Staining Aromatase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-80415] - Analysis in human placenta tissue using Aromatase antibody. Counter stained with hematoxylin.

Flow Cytometry: Aromatase Antibody [NBP2-80415] - Analysis of HepG2 cells with Aromatase antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-80415] - Staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-80415] - Staining Aromatase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Product Discontinued

Aromatase Antibody Summary

• Immunogen: Synthetic peptide within Human Aromatase aa 91-140 / 503. (SwissProt: P11511 Human; SwissProt: P28649 Mouse)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP19A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aromatase Antibody

• ARO1
• ARO1EC 1.14.14.1
• Aromatase
• AROsubfamily XIX (aromatization of androgens)
• CYAR
• CYARMGC104309
• CYP19
• CYP19A1
• CYPXIX
• cytochrome P450, family 19, subfamily A, polypeptide 1
• Cytochrome P-450AROM
• EC 1.14.14.14
• Estrogen synthase
• microsomal monooxygenase

Background

Aromatase is a member of the cytochrome P450 superfamily, whose function is to produce estrogens by aromatizing androgens. The primary function of Aromatase is to convert androstenedione to estrone and testosterone to estradiol. This protein is also a key enzyme in steroidogenesis, specifically for the biosynthesis of estrogens. Because estrogens also promote certain cancers and other diseases, aromatase inhibitors are frequently used to treat such diseases. It also plays an important role in sexual differentiation, fertility, and carcinogenesis.

Aromatase is localized in the endoplasmic reticulum of the cell and its activity is regulated by tissue specific promoters that are in turn controlled by hormones, cytokines, and other factors. It can be found in many tissues reproductive tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, endometrium as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.

Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. The activity is increased by environmental factors such as age, obesity, insulin, gonadotropins, and alcohol but decreased by prolactin, anti-m llerian hormone, and smoking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP19A1