ARID1B Antibody - BSA Free

Immunohistochemistry: ARID1B Antibody [NB100-57485] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID1B used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.

Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57485] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-ARID1B used at a dilution of 1:250.

Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57485] - Human ovarian carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Immunoprecipitation: ARID1B Antibody [NB100-57485] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20 percent of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57485 used for IP at 6 ug per reaction. For blotting immunoprecipitated ARID1B, NB100-57485 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: ARID1B Antibody [NB100-57485] - Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57485 (lot NB100-57485-2) used for IP at 6 ug per reaction. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-57485-1. For blotting immunoprecipitated ARID1B, NB100-57485 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ARID1B Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2186 and the C-terminus (residue 2286) of human AT rich interactive domain 1B using the numbering given in entry NP_787059.2 (GeneID 57492).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARID1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARID1B Antibody - BSA Free

• 6A3-5
• ARID domain-containing protein 1B
• AT rich interactive domain 1B (SWI1-like)
• BAF250B
• BRG1-associated factor 250b
• BRG1-binding protein ELD/OSA1
• BRG1-binding protein hELD/OSA1
• BRIGHT
• DAN15P250R
• ELD (eyelid)/OSA protein
• ELD/OSA1
• hOsa2
• JPUT1
• KDM5B
• KIAA1235AT-rich interactive domain-containing protein 1B
• Osa homolog 2
• OSA2
• p250R
• PLU1
• RBBP2H1A

Background

ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ARID1B Antibody (NB100-57485). (Showing 1 - 1 of 1 FAQ).

1. What does WB(-) mean? That image was a blot on IP done with the antibody but it did not state whether that the observed IP band was detected by the antibody or the other antibody (NB100-57484). It would be great if you could confirm with Novus on whether the observed band was blotted with NB100-57485 and whether the antibody has been tested with whole cell lysate. Could you please advise the above? Thanks for your help :)
   • Thank you for contacting Novus Biologicals Technical Support. NB100-57485 is not recommended for use in Western blot. It has been tested and validated for use in IP and IHC-Paraffin. The Western blot image you see associate with that product represents an immunoprecipitation of HeLa cell extracts using NB100-57485 and NB100-57484. The antibody that was used for the Western blot was NB100-57484.

Bioinformatics

• Gene Symbol: ARID1B
• Entrez:
  • 57492(Human)
• Uniprot:
  • Q8NFD5(Human)