BRD7 Antibody

Images

 
Western Blot: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (NBP1-28727) ...read more
Immunoprecipitation: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using ...read more

Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BRD7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 601 and 651 of human bromodomain containing 7 using the numbering given in entry NP_037395.2 (GeneID 29117).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 -10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-28727 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRD7 Antibody

  • 75 kDa bromodomain protein
  • BP75
  • BP75bromodomain-containing 7
  • BRD7
  • bromodomain containing 7
  • bromodomain-containing protein 7
  • CELTIX1
  • CELTIX1NAG4
  • NAG4
  • Protein CELTIX-1

Background

Bromodomain-containing protein 7 was identified in a two-hybrid screen as an interactor of interferon regulatory factor-2 (IRF-2) and called CELTIX1. BRD7 was also identified as a binding partner of disheveled-1 (Dvl-1) and found to play a role in Wnt signaling and the regulation of glycogen synthase kinase 3-beta activity. Recently, BRD7 was identified as a subunit of the polybromo-associated BRG1-associated factor (PBAF) complex and shown to be required for the modulation of transcription in embryonic stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRD7 Antibody (NBP1-28727)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BRD7
Entrez
Uniprot