Argininosuccinate Synthase Overexpression Lysate

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Western Blot: Argininosuccinate Synthase Overexpression Lysate (Adult Normal) [NBL1-07779] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Argininosuccinate ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Argininosuccinate Synthase Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223189 and based on accession number NM_000050. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens argininosuccinate synthetase 1 (ASS1), transcript variant 1, mRNA.
Gene
ASS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Argininosuccinate Synthase Overexpression Lysate

  • argininosuccinate synthase 1
  • argininosuccinate synthase
  • argininosuccinate synthetase 1
  • argininosuccinate synthetase
  • ASSCTLN1
  • citrulline-aspartate ligase
  • Citrulline--aspartate ligase
  • EC 6.3.4.5

Background

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer variant. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Argininosuccinate Synthase Lysate (NBL1-07779) (0)

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Video Protocols

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Bioinformatics

Gene Symbol ASS1