Argininosuccinate Synthase Antibody (11F1)

Western Blot: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - Western blot analysis of ASS1 on HepG2 (1) and SiHa (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - Analysis of mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - Flow cytometric analysis of HepG2 cells with ASS1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody (11F1) [NBP2-76941] - Analysis of human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

Product Discontinued

Argininosuccinate Synthase Antibody (11F1) Summary

• Immunogen: Recombinant protein within Human Argininosuccinate Synthase aa 1-412 / 412. (SwissProt: P00966 Human; SwissProt: P16460 Mouse)
• Localization: Cytosol.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Argininosuccinate Synthase Antibody (11F1)

• argininosuccinate synthase 1
• argininosuccinate synthase
• argininosuccinate synthetase 1
• argininosuccinate synthetase
• ASSCTLN1
• citrulline-aspartate ligase
• Citrulline--aspartate ligase
• EC 6.3.4.5

Background

Argininosuccinate Synthase is encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of Argininosuccinate Synthase cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASS1