ARG2 Antibody (2611B) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Arginase 2/ARG2 Monoclonal Antibody (Catalog # MAB10602) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Arginase 2/ARG2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Arginase 2/ARG2 at approximately 40 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Arginase 2/ARG2 Monoclonal Antibody (Catalog # MAB10602) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Product Discontinued

ARG2 Antibody (2611B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived human Arginase 2/ARG2
  Val23-Ile354
  Accession # P78540
• Specificity: Detects human Arginase 2/ARG2 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARG2 Antibody (2611B) [Unconjugated]

• ARG2
• Arginase 2
• arginase, type II
• Arginase-2
• arginase-2, mitochondrial
• EC 3.5.3
• EC 3.5.3.1
• kidney arginase
• Kidney-type arginase
• L-arginine amidinohydrolase
• L-arginine ureahydrolase
• nonhepatic arginase
• Non-hepatic arginase
• Type II Arginase

Background

Arginase-2, a binuclear manganese-dependent metalloenzyme, is an approximately 36 kDa protein that forms a trimeric active molecule that catalyses the conversion of L-arginine into L-ornithine and urea (1). There are two isoforms of human arginase that share 58% sequence identity. Arginases are critical to the urea cycle and also influence downstream nitric oxide and polyamine levels (2) and hence are implicated to play a role in a number of diseases. While Arginase 1 is cytosolic and expressed predominantly in the liver, Arginase 2 is a mitochondrial enzyme expressed in several non-hepatic tissues including kidney, prostate and immune cells where it is regulated by inflammatory cytokines (2,3). Arginase 2 is implicated in regulation of a nitric oxide-dependent inflammatory response in lung and bronchial epithelial disease (4,5).  It has been implicated in neuronal disease such as Huntington's disease due to nitric oxide signaling and suppressed enzymatic activity (6) and to play a role in atherosclerosis and hypertension through signaling pathways independent of its enzymatic activity (7,8). Arginase 2 promotes tumorigenesis through immune suppression, metabolism, cell proliferation and vascularization (9-11). Arginase-2 was noted as an attractive tumor therapeutic target (10) given that genetic deletion in mice does not lead to significant abnormalities (12).

1. Dowling, D. et al. (2008) Cell Mol. Life Sci. 65:2039.
2. Munder, M. (2009) Br. J. Pharmacol. 158:638.
3. Geiger, R. et al. (2016) Cell 167:829.
4. Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
5. Xu, W. et al. (2016) J. Clin. Invest. 126:2465.
6. Bichell, T.J.V. et al. (2017) Biochim. Biophys. Acta 1863:1596.
7. Jin, Y. et al. (2014) Am. J. Physiol. Lung Cell Mol. Physiol. 307:L197.
8. Xiong, Y. et al. (2014) Autophagy 10:2223.
9. Ino, Y. et al. (2013) PLoS One 8:e55146.
10. Zaytouni, T. et al. (2017) Nat. Commun. 8:242.
11. Costa, H. et al. (2016) Oncotarget 7:47221.
12. Shi, O. et al. (2001) Mol. Cell Biol. 21:811.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P78540()