ARAP1 Antibody - BSA Free

Western Blot: ARAP1 Antibody [NB100-68223] - Detection of Human CENTD2/ARAP1 on HeLa whole cell lysate using NB100-68223. CENTD2/ARAP1 was also immunoprecipitated by another rabbit anti-CENTD2/ARAP1 antibody.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ARAP1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1160 and 1210 of human centaurin, delta 2 (ankyrin repeat, and pleckstrin homology domains-containing protein 1) using the numbering given in entry NP_056057.1 (GeneID 116985).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: WB reactivity reported in (PMID: 22573888).

Reactivity Notes

Mouse reactivity reported in (PMID: 22573888).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARAP1 Antibody - BSA Free

• arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
• ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
• centaurin, delta 2
• Centaurin-delta-2
• CENTD2ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containingprotein 1
• cnt-d2
• KIAA0782centaurin-delta-2

Background

The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. The RHO-GAP activity may mediate cell rounding and loss of stress fibers. At least three transcript variants encoding different isoforms have been found for this gene, but the full-length natures of all variants have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARAP1 Antibody (NB100-68223)(2)

Publications using NB100-68223

• Etienne Caron, Romain Roncagalli, Takeshi Hase, Witold E. Wolski, Meena Choi, Marisa G. Menoita, Stephane Durand, Antonio García-Blesa, Ivo Fierro-Monti, Tatjana Sajic, Moritz Heusel, Tobias Weiss, Marie Malissen, Ralph Schlapbach, Ben C. Collins, Samik Ghosh, Hiroaki Kitano, Ruedi Aebersold, Bernard Malissen, Matthias Gstaiger Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry Cell Reports 2017-03-28 [PMID: 28355572]
• Hasegawa J, Tsujita K, Takenawa T, Itoh T. ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5 Mol Biol Cell 2012-07-01 [PMID: 22573888] (WB, Mouse)

Bioinformatics

• Gene Symbol: ARAP1
• Entrez:
  • 116985(Human)
• Uniprot:
  • Q96P48(Human)