APRIL/TNFSF13 Antibody (670820) [Unconjugated]

Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND50 is typically0.15-0.75 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 670820
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

APRIL/TNFSF13 Antibody (670820) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human APRIL/TNFSF13
  Ala105-Leu250
  Accession # O75888
• Specificity: Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse APRIL is observed, and no cross-reactivity with recombinant human BAFF is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF13
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.15-0.75 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APRIL/TNFSF13 Antibody (670820) [Unconjugated]

• A proliferation-inducing ligand
• APRIL
• APRILFLJ57090
• CD256 antigen
• CD256
• TALL2
• TALL-2
• TNF- and APOL-related leukocyte expressed ligand 2
• TNF-related death ligand 1
• TNFSF13
• TRDL1
• TRDL-1
• tumor necrosis factor (ligand) superfamily, member 13
• tumor necrosis factor ligand superfamily member 13
• tumor necrosis factor-like protein ZTNF2
• tumor necrosis factor-related death ligand-1
• ZTNF2

Background

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APRIL/TNFSF13 Antibody (MAB5860)(3)

Publications using MAB5860

• H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Sci Rep, 2020-07-28;10(1):12572. 2020-07-28 [PMID: 32724091] (Neutralization, Human)
• Nurse-like cells promote CLL survival through LFA-3/CD2 interactions Oncotarget, 2016-01-01;0(0):. 2016-01-01 [PMID: 27903981] (Neutralization, Human)
• Osorio C, Chacon P, White M, Kisiswa L, Wyatt S, Rodriguez-Tebar A, Davies A Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci, 2014-01-18;59(0):24-36. 2014-01-18 [PMID: 24444792] (Neutralization, Human)

Bioinformatics

• Gene Symbol: TNFSF13
• Uniprot:
  • O75888(Human)