Aprataxin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Aprataxin Antibody [NBP2-39041] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & nucleoli.

Immunohistochemistry: Aprataxin Antibody [NBP2-39041] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

ChIP-Exo-Seq composite graph for Anti-APTX (NBP2-39041) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aprataxin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Aprataxin Antibody - BSA Free (NBP2-39041) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: HMVNELYPYIVEFEEEAKNPGLETHRKRKRSGNSDSIERDAAQEAEAGTGLEPGSNSGQCSVPLKKGKDAPIKKESLGHWSQGLKISMQDPKMQVYKD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APTX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Aprataxin Protein (NBP2-39041PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Aprataxin Antibody - BSA Free

• AOA1EAOH
• AOAMGC1072
• aprataxin
• ataxia 1, early onset with hypoalbuminemia
• AXA1
• EC 3.-
• EOAHA
• FHA-HIT
• FLJ20157
• Forkhead-associated domain histidine triad-like protein

Background

Aprataxin encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APTX
• Uniprot:
  • Q7Z2E3(Human)