Aprataxin Antibody - BSA Free

Western Blot: Aprataxin Antibody [NB100-534] - Detection of human Aprataxin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: Aprataxin Antibody [NB100-534] - Whole cell lysate (50 ug) from normal human lymphoblasts (C3ABR cells; WT) or lymphoblasts from an AOA patient (738RM cells; AOA). Antibody used at 0.1 and 0.04 ug/ml.

Western Blot: Aprataxin Antibody [NB100-534] - Detection of Human Aprataxin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Aprataxin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Aprataxin Antibody - BSA Free (NB100-534) is a polyclonal antibody validated for use in WB and IP. Anti-Aprataxin Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Aprataxin using the numbering given in SwissProt Entry Q7Z2E3 (GeneID 54840).
• Specificity: NB100-534 is specific for human Aprataxin protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APTX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1 - 4 ug/mg lysate
  • Western Blot
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000. Immunoprecipitation: 1 to 4 ug/mg lysate. Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
• Control:

  UbcH7/UBE2L3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aprataxin Antibody - BSA Free

• AOA1EAOH
• AOAMGC1072
• aprataxin
• ataxia 1, early onset with hypoalbuminemia
• AXA1
• EC 3.-
• EOAHA
• FHA-HIT
• FLJ20157
• Forkhead-associated domain histidine triad-like protein

Background

This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aprataxin Antibody (NB100-534)(5)

Publications using NB100-534

• Chu C, Qu K, Zhong FL et al. Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions. Mol Cell 2011-11-18 [PMID: 21963238]
• Sykora P, Croteau DL, Bohr VA, Wilson DM 3rd. Aprataxin localizes to mitochondria and preserves mitochondrial function. Proc Natl Acad Sci U S A. 2011-04-18 [PMID: 21502511]
• Asai H, Hirano M, Shimada K et al. Protein kinase C gamma, a protein causative for dominant ataxia, negatively regulates nuclear import of recessive-ataxia-related aprataxin. Hum Mol Genet 2009-10-01 [PMID: 19561170]
• Biton S, Dar I, Mittelman L et al. Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells. J Biol Chem 2006-06-01 [PMID: 16627474]
• Luo H, Chan DW, Yang T et al. A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment. Mol Cell Biol 2004-10-01 [PMID: 15367657]

Bioinformatics

• Gene Symbol: APTX
• Entrez:
  • 7332(Human)
• Uniprot:
  • Q7Z2E3(Human)