Aprataxin Antibody

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Western Blot: Aprataxin Antibody [NB100-534] - Detection of Human Aprataxin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Western Blot: Aprataxin Antibody [NB100-534] - Whole cell lysate (50 ug) from normal human lymphoblasts (C3ABR cells; WT) or lymphoblasts from an AOA patient (738RM cells; AOA). Antibody used at 0.1 and 0.04 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

Aprataxin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Aprataxin using the numbering given in SwissProt Entry Q7Z2E3 (GeneID 54840).
Specificity
NB100-534 is specific for human Aprataxin protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APTX
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Immunoprecipitation 1 - 4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000. Immunoprecipitation: 1 to 4 ug/mg lysate. Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
UbcH7/UBE2L3 Lysate (NBL1-17537)
Publications
Read Publications using NB100-534.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aprataxin Antibody

  • AOA1EAOH
  • AOAMGC1072
  • aprataxin
  • ataxia 1, early onset with hypoalbuminemia
  • AXA1
  • EC 3.-
  • EOAHA
  • FHA-HIT
  • FLJ20157
  • Forkhead-associated domain histidine triad-like protein

Background

This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for Aprataxin Antibody (NB100-534)(5)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol APTX
Entrez
Uniprot