APP Antibody

Product Discontinued

APP Antibody Summary

• Immunogen: A synthetic peptide (HMNVQNGKWESDPSGTKTC, aa: 44-62) as part of mouse APP isoform A conjugated to the immunogenic protein Blue Carrier Protein
• Localization: Accession Number: APP_MOUSE
• Specificity: Specificity for APP was confirmed by IHC.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APP
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:3000
• Application Notes: Immunohistochemistry.Recommended to be used at a dilution of 1:500 to 1:3000. This antiserum has not yet been tested for western blot. The optimal working dilution should be determined by the end user.

Reactivity Notes

Reacts with rat. Reactivity with other species has not yet been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water. Centrifuge to remove any insoluble material.

Alternate Names for APP Antibody

• amyloid beta (A4) precursor protein-binding, family B, member 2
• amyloid beta A4 precursor protein-binding family B member 2
• Amyloid beta precursor protein
• Amyloid beta
• APP
• beta Amyloid
• Protease Nexin II

Background

FUNCTION: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity). FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1 inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues: kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, all the isoforms are abundantly expressed in Purkinje cells.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APP Antibody (R-071-100)(3)

Publications using R-071-100

• Guenette SY. Neuromolecular Med. 4: 147-160. 2003-01-01 [PMID: 14716023]
• Andreasen N, Sjogren M, Blennow K. CSF markers for Alzheimer's disease: total tau, phospho-tau and Abeta42. World J Biol Psychiatry. 4(4):147-55. 2003-10-01 [PMID: 14608585]
• Wilson CA, Doms RW, Lee VM. Distinct presenilin-dependent and presenilin-independent gamma-secretases are responsible for total cellular Abeta production. J Neurosci Res. 74(3):361-9. 2003-11-01 [PMID: 14598312]

Bioinformatics

• Gene Symbol: APP
• Entrez:
  • 54226(Rat)