Apolipoprotein L1 Antibody (SR1743) - BSA Free

Western Blot: Apolipoprotein L1 Antibody (SR1743) [NBP3-21988] - Western blot analysis using NBP3-21988 in Human plasma cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IP
• Clone: SR1743
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein L1 Antibody (SR1743) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Apolipoprotein L1 (Uniprot #: O14791)
• Specificity: Detects endogenous levels of total Apolipoprotein L1
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: APOL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Apolipoprotein L1 Antibody (SR1743) - BSA Free

• APOL
• APO-L
• APOL1
• APO-L1
• APO-LI
• APOL-I
• Apolipoprotein L
• apolipoprotein L, 1
• Apolipoprotein L1
• Apolipoprotein L-I
• FSGS4

Background

Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.1- 13.1 within a 127,000-bp region . apoL has been characterized as a pancreas specific, 383-amino acid, 42 kDa protein that contains a 12-amino acid secretory signal peptide. The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol. apoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOL1