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non-muscle Myosin IIA Antibody

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Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Paraffin-embedded human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500.
Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000
Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell ...read more
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Paraffin-embedded mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400.
Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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non-muscle Myosin IIA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.
Predicted Species
Bovine (99%), Chicken (94%), Canine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYH9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
227 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-31733 in the following applications:

  • WB
    1 publication

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for non-muscle Myosin IIA Antibody

  • Cellular myosin heavy chain, type A
  • DFNA17
  • EPSTS
  • FTNSNMMHC-IIA
  • MGC104539
  • MHANMMHC II-a
  • MYH9 variant protein
  • Myosin heavy chain 9
  • Myosin heavy chain, non-muscle IIa
  • myosin, heavy chain 9, non-muscle
  • myosin, heavy polypeptide 9, non-muscle
  • myosin-9
  • NMHC-II-A
  • NMMHCA
  • NMMHC-A
  • Non-muscle myosin heavy chain A
  • Non-muscle myosin heavy chain IIa
  • nonmuscle myosin heavy chain II-A
  • non-muscle myosin heavy polypeptide 9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Apolipoprotein L1 Antibody [NBP1-89033] - Analysis in human cell lines A-431 and MCF-7 using anti-APOL1 antibody. Corresponding APOL1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis. The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins. Diabetes-related changes in the protein composition and the biomechanical properties of human retinal vascular basement membranes. PLoS One (2017)
Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
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Western Blot: Myosin heavy chain 2 Antibody [NBP2-94624] - Analysis of extracts of various cell lines, using Myosin heavy chain 2.Exposure time: 180s.
Immunocytochemistry/Immunofluorescence: Myosin heavy chain 2 Antibody [NBP2-94624] - Analysis of mouse skeletal muscle using Myosin heavy chain 2. Blue: DAPI for nuclear staining.
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Western Blot: CYLD Antibody [NB100-56461] - Analysis of CYLD in HeLa cell lysate using this antibody.
Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using this antibody at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
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Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(6 Reviews)
(51 Publications)
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Applications: WB, IHC, ICC
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Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NRAMP1/SLC11A1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for NRAMP1/SLC11A1 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HEK293 human embryonic kidney cell line transfected with either (A) human NRAMP1/SLC11A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
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Immunohistochemistry-Paraffin: SMARCA6 Antibody [NBP2-38986] - Analysis in human testis and pancreas tissues. Corresponding HELLS RNA-seq data are presented for the same tissues.
Western Blot: SMARCA6 Antibody [NBP2-38986] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/Immunofluorescence: SMARCA6 Antibody [NBP2-38986] - Staining of human cell line MCF7 shows localization to nucleus, the Golgi apparatus & vesicles. Antibody staining is shown in green.
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Applications: WB, ICC/IF, IHC, IHC-P

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Western Blot: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Staining BCHE in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Butyrylcholinesterase/BCHE Antibody [NBP2-89134] - Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Butyrylcholinesterase/BCHE antibody. Counter stained with hematoxylin.
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Western Blot: MASA Antibody [NBP2-13961] - Analysis in control (vector only transfected HEK293T lysate) and ENOPH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: MASA Antibody [NBP2-13961] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.
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Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257)
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-53246] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
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Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.Lane 1: ACTN4 transfected lysate(104.9 KDa).Lane 2: Non-transfected lysate.
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Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Analysis in human cell lines HEK293 and PC-3 using anti-MYH10 antibody. Corresponding MYH10 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
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Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-38877] - Analysis in human cerebral cortex and liver tissues using NBP2-38877 antibody. Corresponding NHE6/SLC9A6 RNA-seq data are presented for the same tissues.
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Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
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Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Immunohistochemistry-Paraffin: Uromodulin Antibody (10.32) [NBP1-50321] - Human kidney tissue, staining in kidney tubules. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.
Immunohistochemistry-Paraffin: Uromodulin Antibody (10.32) [NBP1-50321] - Analysis of anti-UMOD / Uromodulin antibody with human kidney at concentration 20 ug/ml.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for non-muscle Myosin IIA Antibody (NBP1-31733)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol MYH9
Entrez
Uniprot