non-muscle Myosin IIA Antibody

Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000

Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using MYH9 antibody [N1-2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [N1-2], N-term (NBP1-31733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

non-muscle Myosin IIA Antibody Summary

• Description: Novus Biologicals Rabbit non-muscle Myosin IIA Antibody (NBP1-31733) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-non-muscle Myosin IIA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary.
• Predicted Species: Bovine (99%), Chicken (94%), Canine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 227 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for non-muscle Myosin IIA Antibody

• Cellular myosin heavy chain, type A
• DFNA17
• EPSTS
• FTNSNMMHC-IIA
• MGC104539
• MHANMMHC II-a
• MYH9 variant protein
• MYH9
• Myosin heavy chain 9
• Myosin heavy chain, non-muscle IIa
• myosin, heavy chain 9, non-muscle
• myosin, heavy polypeptide 9, non-muscle
• myosin-9
• NMHC-II-A
• NMMHC II-a
• NMMHCA
• NMMHC-A
• NMMHC-IIA
• Non-muscle myosin heavy chain A
• Non-muscle myosin heavy chain IIa
• nonmuscle myosin heavy chain II-A
• non-muscle myosin heavy polypeptide 9
• Non-muscle Myosin IIA

Background

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for non-muscle Myosin IIA Antibody (NBP1-31733)(1)

Publication using NBP1-31733

• Yoon Y, Go G, Yun C et al. Knockdown of CK2 alpha reduces P-cresol-induced fibrosis in human renal proximal tubule epithelial cells via the downregulation of profilin-1 Int. J. Med. Sci. 2020-10-28 [PMID: 33162813] (WB, Human)

Bioinformatics

• Gene Symbol: MYH9
• Entrez:
  • 4627(Human)
  • 17886(Mouse)
• Uniprot:
  • P35579(Human)