Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free

Western Blot: Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free [NBP2-70186] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apolipoprotein H.

Immunohistochemistry: Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free [NBP2-70186] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Western Blot: Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free [NBP2-70186] - Analysis of extracts (10ug) from 5 different cell lines and 4 human tissues(1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)

Immunohistochemistry: Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free [NBP2-70186] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free [NBP2-70186] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI4A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 120-345 of human APOH(NP_000033) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 36.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Apolipoprotein H/ApoH Antibody (OTI4A11) - Azide and BSA Free

• Activated protein C-binding protein
• Anticardiolipin cofactor
• APC inhibitor
• APOH
• apo-H
• apolipoprotein H (beta-2-glycoprotein I)
• Apolipoprotein H
• B2G1
• B2GP1
• B2GPI
• Beta
• beta(2)GPI
• beta-2-glycoprotein 1
• Beta-2-glycoprotein I
• BG

Background

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOH