Apolipoprotein A5 Antibody - BSA Free

Western Blot: Apolipoprotein A5 Antibody [NBP3-03567] - Western blot analysis of extracts of Mouse liver, using Apolipoprotein A5 antibody (NBP3-03567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein A5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Apolipoprotein A5 Antibody - BSA Free (NBP3-03567) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 207-366 of human Apolipoprotein A5 (NP_443200.2). QELHRSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEEVRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWEDITHSLHDQGHSHLGDP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOA5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Apolipoprotein A5 Antibody - BSA Free

• APOAV
• apo-AV
• APOA-V
• Apolipoprotein A5
• apolipoprotein A-V
• FLJ97995
• MGC126836
• RAP3MGC126838
• Regeneration-associated protein 3

Background

Apolipoprotein A5 (ApoA5) is a minor apolipoprotein, predominantly associated with HDL and and occasionally with VLDL and chylomicrons. ApoA-5 is a useful indicator of plasma triglyceride levels, a major risk factor for coronary artery disease.

Apo A5 is expressed in the plasma and the liver, where it is involved in the early phase of liver regeneration.

APOA5 mutations can lead to increaased susceptibility to familial hypertriglyceridemia (FHTR), a common inherited disorder where VLDL concentrations are elevated in the plasma. This disorder increases the risk of heart disease, obesity, and pancreatitis. APOA5 mutations can also cause hyperlipoproteinemia type 5 (HLPP5), characterized by increased amounts of chylomicrons/VLDL and decreased amounts of LDL/HDL in the plasma. This disorder often leads to a wide array of syndromes, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A

ApoA5 antibodies are useful for studying lipoprotein processing and a variety of cardiac diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOA5