Apolipoprotein A-IV/ApoA4 Antibody

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-06019] - Staining of Mouse Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Mu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein A-IV/ApoA4 Antibody Summary

• Immunogen: Peptide with sequence C-QETERVKEEIKKELED corresponding to internal region according to NP_031494.2.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments in mouse and rat liver lysates gave no specific signal but low background. IHC-P: Mouse colon shows staining of cells between the villi, consistent with the observations with the related NB100-57089.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody

• apo-AIV
• apoA-IV
• Apolipoprotein A4
• Apolipoprotein AIV
• Apolipoprotein A-IV
• MGC142154
• MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein A-IV/ApoA4 Antibody (NBP1-06019)(2)

Publications using NBP1-06019

• Reglodi D, Jungling A, Longuespee R et al. Accelerated pre-senile systemic amyloidosis in PACAP knockout mice - a protective role of PACAP in age-related degenerative processes J. Pathol. 2018-05-17 [PMID: 29774542] (IF/IHC, Mouse)
• Orso E, Moehle C, Boettcher A et al. The satiety factor apolipoprotein A-IV modulates intestinal epithelial permeability through its interaction with alpha-catenin: implications for inflammatory bowel diseases. Horm Metab Res 2007-08-01 [PMID: 17712726]

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (NBP1-06019). (Showing 1 - 1 of 1 FAQs).

1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
   • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Bioinformatics

• Gene Symbol: APOA4
• Entrez:
  • 11808(Mouse)
• Uniprot:
  • P06728(Mouse)