Apolipoprotein A-IV/ApoA4 Antibody

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Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NB100-57089] - Staining of Human Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Apolipoprotein A-IV/ApoA4 Antibody Summary

Immunogen
Peptide with sequence C-KEKESQDKTLSLP corresponding to internal region (near C-Terminus) according to NP_000473.2.
Epitope
KEKESQDKTLSLP
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 45.4kDa according to NP_000473.2).
Publications
Read Publication using NB100-57089.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-IV/ApoA4 Antibody (NB100-57089)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (NB100-57089). (Showing 1 - 1 of 1 FAQ).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Secondary Antibodies

 

Isotype Controls

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Diseases for Apolipoprotein A-IV/ApoA4 Antibody (NB100-57089)

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Bioinformatics

Gene Symbol APOA4
Entrez
Uniprot