Apolipoprotein A-IV/ApoA4 Antibody (988829) [Unconjugated]

Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Apolipoprotein A‑IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human Apolipoprotein A‑IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 988829
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Apolipoprotein A-IV/ApoA4 Antibody (988829) [Unconjugated] Summary

• Immunogen: E. coli-derived human Apolipoprotein A-IV/ApoA4 protein
  Glu21-Ser396
  Accession # P06727
• Specificity: Detects human Apolipoprotein A‑IV/ApoA4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody (988829) [Unconjugated]

• apo-AIV
• apoA-IV
• Apolipoprotein A4
• Apolipoprotein AIV
• Apolipoprotein A-IV
• MGC142154
• MGC142156

Background

Apolipoprotein A-IV (ApoA4) is a 45 kDa glycoprotein of the lipid transport system. Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption. Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis (1). ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well (2). ApoA4 shares several structural characteristics with ApoA1 and other exchangeable apolipoproteins. The core domain of human ApoA4 contains thirteen 22-amino acid tandem repeats, and nine of which are predicted to be amphipathic alpha -helical repeats that are critical for lipid binding and self-association (3). The overall structure of a long rod‑like dimer consisting of two 4-helix bundles stacked end-to-end in opposing orientations (4). Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

1. Duverger N. et al. (1991) Eur. J. Biochem. 201:373.
2. Maeda, N. et al. (1994) J. Biol. Chem. 269:23610.
3. Segrest, J. P. et al. (1994). Adv. Protein. Chem. 45:303.
4. Deng, X. et al. (2012) Structure 20:767.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (MAB8125). (Showing 1 - 1 of 1 FAQ).

1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
   • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Bioinformatics

• Uniprot:
  • P06727()