AOF1 Antibody

Immunocytochemistry/Immunofluorescence: AOF1 Antibody [NBP1-90148] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: AOF1 Antibody [NBP1-90148] - Staining of human liver shows nuclear and cytoplasmic positivity in hepatocytes.

Product Discontinued

AOF1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CEQLGISMHKFGERCDLIQEGGRITDPTIDKRMDFHFNALLDVVSEWRKDKTQLQDVPLGEKIEEIYKAFIKESGIQFSELEGQVLQFHLSNLE
• Predicted Species: Mouse (94%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. WB reported in (PMID: 24924415).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for AOF1 Antibody

• AOF1
• bA204B7.3
• C6orf193
• dJ298J15.2
• DKFZp686I0412
• EC 1.14.99.66
• flavin containing 1
• flavin-containing amine oxidase domain-containing protein 1
• FLJ33898
• FLJ34109
• FLJ43328
• KDM1B
• LSD2
• lysine (K)-specific demethylase 1B
• lysine-specific histone demethylase 1B
• lysine-specific histone demethylase 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AOF1 Antibody (NBP1-90148)(1)

Publication using NBP1-90148

• Katz TA, Vasilatos SN, Harrington E et al. Inhibition of histone demethylase, LSD2 (KDM1B), attenuates DNA methylation and increases sensitivity to DNMT inhibitor-induced apoptosis in breast cancer cells Breast Cancer Res. Treat. 2014-07-01 [PMID: 24924415] (WB, Human)
  
  Details:
  Antibody used for WB on MDA-MB-231 cells (scramble control and LSD2-Knock Down) nuclear extracts (Figure 1A)

Bioinformatics

• Gene Symbol: KDM1B
• Entrez:
  • 221656(Human)
• Uniprot:
  • Q8NB78(Human)