ANP32B Antibody (8E10) - Azide and BSA Free

Western Blot: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10. Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of monoclonal antibody to ANP32B on HeLa cell. Antibody concentration 10 ug/ml

ELISA: ANP32B Antibody (8E10) [H00010541-M05] - Detection limit for recombinant GST tagged ANP32B is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 8E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ANP32B Antibody (8E10) - Azide and BSA Free Summary

• Immunogen: ANP32B (NP_006392.1, 1 a.a. ~ 251 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD
• Specificity: This product is specific for Human ANP32B monoclonal antibody (M05), clone 8E10 [Gene ID: 10541].
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANP32B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Mouse monoclonal antibody raised against a full-length recombinant ANP32B.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ANP32B Antibody (8E10) - Azide and BSA Free

• acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• acidic leucine-rich nuclear phosphoprotein 32 family member B
• Acidic protein rich in leucines
• APRILSilver-stainable protein SSP29
• Pal31
• PHAPI2SSP29
• Putative HLA-DR-associated protein I-2

Background

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANP32B
• Uniprot:
  • NP_006392.1(Human)