Annexin A8/ANXA8 Antibody

Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Annexin A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Annexin A8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Annexin A8 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Annexin A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8105) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Annexin A8/ANXA8 Antibody Summary

• Immunogen: E. coli-derived recombinant human Annexin A8/ANXA8
  Ala2-Pro327
  Accession # P13928
• Specificity: Detects human Annexin A8/ANXA8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) ANXA4, rhANXA5, rhANXA6, rhANXA7, rhANXA11, and rhANXA13A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ANXA8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A8/ANXA8 Antibody

• Annexin A8
• Annexin VIII
• Annexin-8
• Anx8
• ANXA8
• ANXA8L2
• FLJ53095
• VAC-beta
• Vascular anticoagulant-beta

Background

Annexin A8 (ANXA8), also known as VAC-beta, is an approximately 37 kDa member of the Annexin family of calcium-dependent phospholipid-binding proteins. Annexin A8 associates with the cytosolic face of endosomal membranes in the presence of elevated calcium, and it plays a role in endosomal vesicle trafficking. Annexin A8 is widely expressed in epithelial cells, particularly in the tongue, skin, cornea, and pubertal mammary ducts. It is upregulated in mammary hyperplasia and adenocarcinoma as well as infiltrating pancreatic adenocarcinoma. Annexin A8 is also upregulated during osteoclast differentiation at sites of bone loss. A highly similar duplicated copy of this gene is found in close proximity on the long arm of chromosome 10. Also, multiple copies of ANXA8-like sequences are reported on human chromosome 10q11. Human Annexin A8 shares 92% amino acid sequence identity with mouse and rat Annexin A8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A8/ANXA8 Antibody (AF8105)(1)

Publication using AF8105

• G. Kenneth Gray, Carman Man-Chung Li, Jennifer M. Rosenbluth, Laura M. Selfors, Nomeda Girnius, Jia-Ren Lin et al. A human breast atlas integrating single-cell proteomics and transcriptomics Developmental Cell 2022-06-01 [PMID: 35617956]

Bioinformatics

• Gene Symbol: ANXA8
• Uniprot:
  • P13928()