Annexin A4 Antibody - BSA Free

Western Blot: Annexin A4 Antibody [NBP2-15409] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Annexin IV antibody, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody [NBP2-15409] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Annexin A4 Antibody [NBP2-15409] - D54MG xenograft. Annexin IV antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: Annexin A4 Antibody [NBP2-15409] - Immunoprecipitation of Annexin IV protein from HepG2 whole cell extracts using 5 ug of Annexin IV antibody. Western blot analysis was performed using Annexin IV antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Annexin A4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Annexin A4. The exact sequence is proprietary.
• Predicted Species: Mouse (91%), Rat (92%), Porcine (96%), Bovine (94%), Rhesus Macaque (98%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANXA4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Annexin A4 Antibody - BSA Free

• AIV
• Annexin A4
• annexin IV (placental anticoagulant protein II)
• Annexin IV
• annexin-4
• ANX4
• ANX4Carbohydrate-binding protein p33/p41
• ANXA4
• Chromobindin-4
• chromobindin-4,35-beta calcimedin
• DKFZp686H02120
• Endonexin I
• Lipocortin IV
• MGC75105
• P32.5
• PAP-II
• PIG28
• Placental anticoagulant protein II
• PP4-X
• proliferation-inducing gene 28
• proliferation-inducing protein 28
• Protein II
• ZAP36

Background

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for Annexin A4 Antibody (NBP2-15409) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IP Human 09/15/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: Platelet Lysates
• Species: Human
• Lot: 40184

Bioinformatics

• Gene Symbol: ANXA4
• Uniprot:
  • P09525()