Annexin A3 Antibody (693510) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Annexin A3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 693510
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Annexin A3 Antibody (693510) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Annexin A3
  Ala2-Asp323
  Accession # P12429
• Specificity: Detects human Annexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Annexin A1, A2, A4, A6, A8, A9, A10, A11, or A13 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A3 Antibody (693510) [Unconjugated]

• 35-alpha calcimedin
• Annexin A3
• annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase
• Annexin III
• annexin-3
• ANXA3
• Calcimedin 35-alpha
• calcimedin 35-alpha
• Lipocortin III
• PAP-III
• Placental anticoagulant protein III
• placental anticoagulant protein III, calcimedin 35-alpha

Background

Annexin A3 (ANXA3), also known as Annexin III and Lipocortin III (LPC3), is a 36 kDa member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing calcium-dependent phospholipid-binding sites. The C-terminal domain is comprised of four 60-70 amino acid (aa) annexin repeats. Annexin A3 is upregulated in microglia following stroke and in parenchymal hepatocytes in regenerating liver. It can be up- or down‑regulated in various carcinomas. Alternate splicing generates an additional isoform with a 30 amino acid (aa) deletion near the N-terminus. Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA3
• Uniprot:
  • P12429()