Anillin Antibody (CL0303) - BSA Free

Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Lane 1: Marker [kDa] Lane 2:Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in U251 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.

Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Interaction of Anillin with GST-fused protein: MDA-MB-231 cells were lysed in TBS, pH 7.5, containing 0.75% Triton X-100 with inhibitors of proteases and phosphatases. Lysate was sonicated and cleared by centrifugation. for the input, sample buffer was added to part of the lysate and heated fro 10min 95 C. The rest of the lysate was incubated with either GST or GST-fused protein immobilized on agarose beads for 2h in 4 C. the beads were washed three times in lysis buffer, resuspended in sample buffer and heated for 10min 95 C. The samples were resolved using SDS-PAGE and immunoblotted with anti-ANLN antibody. This image was submitted by customer review.

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in A431 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in HeLa cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in MCF7 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in U2OS cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in a subset of tumor cells.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL0303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Anillin Antibody (CL0303) - BSA Free Summary

• Description: Novus Biologicals Mouse Anillin Antibody (CL0303) - BSA Free (NBP2-52908) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9NQW6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Anillin Recombinant Protein Antigen (NBP2-52908PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Anillin Antibody (CL0303) - BSA Free

• actin-binding protein anillin
• anillin (Drosophila Scraps homolog), actin binding protein
• ANILLIN
• anillin, actin binding protein (scraps homolog, Drosophila)
• anillin, actin binding protein
• DKFZp779A055
• Drosophila Scraps homolog
• scra
• Scraps

Background

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Anillin Antibody (NBP2-52908) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/15/2018

Summary

• Application: Western Blot
• Sample Tested: GST-pull down assay
• Species: Human
• Lot: 02616

Bioinformatics

• Gene Symbol: ANLN