Anillin Antibody (CL0303) - Azide and BSA Free

Staining in A431 cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Analysis in human testis and skeletal muscle tissues using NBP3-43945 antibody. Corresponding Anillin RNA-seq data are presented for the same tissues.

Staining in U251 cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in MCF7 cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Lane 1: Marker [kDa]Lane 2:Human cell line U-251 MG

Staining in HeLa cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U2OS cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining of human colorectal cancer shows moderate to strong nuclear positivity in a subset of tumor cells.

Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.

Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Anillin antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Anillin Antibody (CL0303) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Anillin Antibody (CL0303) - Azide and BSA Free (NBP2-52908) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9NQW6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Anillin Antibody (CL0303) - Azide and BSA Free

• actin-binding protein anillin
• anillin (Drosophila Scraps homolog), actin binding protein
• ANILLIN
• anillin, actin binding protein (scraps homolog, Drosophila)
• anillin, actin binding protein
• DKFZp779A055
• Drosophila Scraps homolog
• scra
• Scraps

Background

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANLN