Anillin Antibody (CL0301) - BSA Free

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of U-251 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of A-431 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human lung cancer shows moderate to strong nuclear positivity in a subset of tumor cells.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Anillin Antibody (CL0301) - BSA Free Summary

• Description: Novus Biologicals Mouse Anillin Antibody (CL0301) - BSA Free (NBP2-52907) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Anillin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9NQW6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  Anillin Recombinant Protein Antigen (NBP2-52907PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Anillin Antibody (CL0301) - BSA Free

• actin-binding protein anillin
• anillin (Drosophila Scraps homolog), actin binding protein
• ANILLIN
• anillin, actin binding protein (scraps homolog, Drosophila)
• anillin, actin binding protein
• DKFZp779A055
• Drosophila Scraps homolog
• scra
• Scraps

Background

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Anillin Antibody (NBP2-52907)(1)

Publication using NBP2-52907

• Johnson KO, Harel L, Triplett JW., et Al. Postsynaptic NMDA Receptor Expression Is Required for Visual Corticocollicular Projection Refinement in the Mouse Superior Colliculus J Neurosci 2023-01-30 [PMID: 36717228]

Bioinformatics

• Gene Symbol: ANLN