Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clone: 766544
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant mouse Angiopoietin-like Protein 6/ANGPTL6
  Ala25-Leu457
  Accession # Q8ROZ6
• Specificity: Detects mouse Angiopoietin-like Protein 6/ANGPTL6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Angiopoietin-like 6 or recombinant mouse Angiopoietin-like 2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Alexa Fluor® 488]

• AGF
• AGFAngiopoietin-related protein 5
• Angiopoietin like Protein 6
• angiopoietin-like 6
• Angiopoietin-like Protein 6
• Angiopoietin-related growth factor
• ANGPTL6
• ARP5
• ARP5angiopoietin-related protein 6

Background

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.