Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

ELISA: Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) [NBP2-68215] - Samples were spiked with high concentrations of Rat Angiopoietin-like Protein 3/ANGPTL3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) [NBP2-68215] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL

Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat ANGPTL3 in samples. No significant cross-reactivity or interference between Rat ANGPTL3 and analogues was observed.
• Standard Curve Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.09%
• Intra-Assay: CV% < 5.09%
• Spike Recovery: 90-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ANGPTL3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

• AGNPT5
• Ang-5
• angiopoietin 5
• Angiopoietin-5
• angiopoietin-like 3
• Angiopoietin-like Protein 3
• angiopoietin-related protein 3
• ANGPT5angiopoietin-5
• ANGPTL3
• FHBL2

Background

ANGPTL3 is a 460 amino acid protein that is found most commonly in the liver, but has also been shown to reside in kidney samples, and contains three domains consisting of a signal peptide, N-terminal coiled-coil and C-terminal Fibrinogen (FBN) like domain. This protein is involved with the lipid metabolism disease pathways in relation to low levels of LDL (low density lipoproteins), HDL (High density lipoproteins), and triglycerides in plasma. This protein plays a role in angiogenesis and has been shown to interact with ITGAV, ITGB3, and ITGA5, and work is being done to study the protein and the related hypobetalipoproteinemia and atherosclerosis involvement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (NBP2-68215). (Showing 1 - 1 of 1 FAQ).

1. When using Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) NBP2-68215, what would be the dilution factor for wild type rat serum samples?
   • Our team diluted the samples 200-1000 fold and they have found the conentrations of Angiopoietin-like Protein 3/ANGPTL3 in rat serum to be in a range 85.2-498.5ng/ml. I have also checked other ELISA kits (with similar detection range) for mouse and human serum samples and in these they report diluting the samples 100 and 50 fold respectively.

Bioinformatics

• Gene Symbol: ANGPTL3