Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free

Western Blot: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPTL1 .

Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: OTI1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 24-491 of human ANGPTL1(NP_004664) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPTL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 54.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free

• ANG-3
• ANG3angioarrestin
• Angiopoietin like Protein 1
• angiopoietin Y1
• angiopoietin-3
• angiopoietin-like 1
• Angiopoietin-like Protein 1
• angiopoietin-related protein 1
• ANGPT3
• ANGPTL1
• AngY
• AngY1
• ARP1
• ARP1angiopoietin 3
• dJ595C2.2 (angiopoietin Y1)
• dJ595C2.2
• KIAA0351
• UNQ162

Background

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPTL1