Angiopoietin-4 Antibody (156215)

Angiopoietin-4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human Angiopoietin-4 Monoclonal Antibody (Catalog # MAB964) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 156215
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Angiopoietin-4 Antibody (156215) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-4
  Met1-Ile503
  Accession # Q9Y264
• Specificity: Detects human Angiopoietin-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Ang‑1, rhAng‑2, rhAng-X, or rhANGPTL3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPT4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-4 Antibody (156215)

• AGP4
• ANG3
• ANG-3
• ANG-4
• ANG4angiopoietin-3
• angiopoietin 4
• Angiopoietin-3
• Angiopoietin-4
• ANGPT4
• dJ824F16.2 (angiopoietin 4)
• MGC138181
• MGC138183

Background

Human Angiopoietin-4 (Ang-4) (1), alternatively named Ang-3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47%, and 54% aa sequence identity with human Ang-1, human Ang-2, and mouse Ang‑3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang‑3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-4 Antibody (MAB964)(1)

Publication using MAB964

• M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis Oncogene, 2022-06-14;0(0):. 2022-06-14 [PMID: 35701366] (Simple Western, Xenograft)

Bioinformatics

• Gene Symbol: ANGPT4
• Uniprot:
  • Q9Y264()