Angiopoietin-3 Antibody

Angiopoietin‑3 was detected in perfusion fixed frozen sections of rat kidney using 5 µg/mL Goat Anti-Mouse Angiopoietin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF738) overnight at4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiopoietin-3 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Angiopoietin-3
• Specificity: Detects mouse Angiopoietin-3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin‑2 or rhAngoipoietin-3 was observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Angpt4
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-3 Antibody

• ANG3
• ANG4
• Angiopoietin-3
• ANGPT3

Background

Mouse angiopoietin-3 (Ang-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse Ang-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46%, and 54% aa sequence identity with mouse Ang-1, mouse Ang-2, and human Ang-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang-3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (1, 2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Angpt4