Human Angiogenin ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. ANG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human ANG. |
| Standard Curve Range |
78 to 5000 pg/mL |
| Sensitivity |
12 pg/mL |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
ANG |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- ABC diluent buffer: 12 ml.
- Antibody diluent buffer: 12 ml.
- Avidin-Biotin-Peroxidase Complex (ABC): 130 µl, dilution 1:100.
- Biotinylated anti- human ANG antibody: 130 µl, dilution 1:100.
- Lyophilized recombinant human ANG standard: 5 ng/tube×2.
- One 96-well plate precoated with anti- human ANG antibody.
- Sample diluent buffer: 30 ml.
- TMB color developing agent: 10 ml.
- TMB stop solution: 10 ml.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Angiogenin ELISA Kit (Colorimetric)
Background
Angiogenin was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human recombinant angiogenin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Angiogenin ELISA Kit (KA1252). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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