Angiogenin Antibody (14017)

Product Discontinued

Angiogenin Antibody (14017) Summary

• Immunogen: purified recombinant human angiogenin expressed in E. coli.
• Localization: Secreted
• Specificity: Angiogenin (14017.7)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-8 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: Use at a concentration of 1 - 2 ug/ml. WB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 14.4 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Angiogenin Antibody (14017)

• ALS9
• ANG
• Angiogenin
• angiogenin, ribonuclease, RNase A family, 5
• EC 3.1.27
• EC 3.1.27.-
• epididymis luminal protein 168
• HEL168
• MGC22466
• MGC71966
• Ribonuclease 5
• RNase 5
• RNASE5RNASE4

Background

Angiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence identity with pancreatic RNase. Like other members of the ribonuclease superfamily, angiogenin is a cytotoxic agent that can eliminate cellular protein synthesis. Angiogenin causes protein synthesis inhibition by functioning as a cytotoxic tRNA-specific RNase. Angiogenesis is the formation of blood vessels or capillaries from existing blood vessels due to specific signals. Angiogenin induces the growth of new blood vessels. It stimulates capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin exhibits both angiogenic and non-angiogenic activities and is involved in endothelial cell migration, proliferation, and differentiation. It is produced by a variety of tumor and normal cell types. Cells that express angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, normal colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. Angiogenin is also present in normal human plasma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANG
• Entrez:
  • 283(Human)