Androgen R/NR3C4 Antibody

Product Discontinued

Androgen R/NR3C4 Antibody Summary

• Immunogen: Fusion protein containing residues 331-572 of human androgen receptor.
• Localization: Nuclear
• Specificity: This detects Androgen Receptor. It does not detect estrogen, progesterone, or glucocorticoid receptors.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AR
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Use in Chromatin Immunoprecipition was reported in the scientific literature (PMID: 17505060). Immunohistochemical staining of AR in rat brain results in intense nuclear staining. For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer, pH 7.4 is recommended.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Androgen R/NR3C4 Antibody

• AIS
• Androgen R
• androgen receptor
• AndrogenR
• AR
• DHTR
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• NR3C4
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle. The corresponding gene for the androgen receptor is NR3C4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Androgen R/NR3C4 Antibody (NB300-551)(10)

Publications using NB300-551

• de Souza BR, de Almeida Chuffa LG, Simao VA, Camargo ICC. Histopathological changes in androgenized ovaries are recovered by melatonin treatment. Int J Exp Pathol 2018-08-01 [PMID: 30256483] (IF/IHC, Rat)
• Andrade G, Simao V, Souza B et al. Sex steroid receptors profiling is influenced by nandrolone decanoate in the ampulla of the fallopian tube: Post-treatment and post-recovery analyses Tissue and Cell 2018-02-01 [PMID: 29429521] (IHC-P, Rat)
• Simao VA, Chuffa LG, Camargo IC. Ovarian sex steroid receptors and sex hormones in androgenized rats. Reproduction 2016-08-25 [PMID: 27562815] (IF/IHC)
• Ortega HH, Salvetti NR, Padmanabhan V et al. Developmental programming: prenatal androgen excess disrupts ovarian steroid receptor balance. Reproduction. 2009-05-01 [PMID: 19261835]
• Truica CI, Byers S, Gelmann EP et al. Beta-catenin affects androgen receptor transcriptional activity and ligand specificity. Cancer Res. 2000-09-01 [PMID: 10987273]
• Bello D, Webber MM, Kleinman HK et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis. 1997-06-01 [PMID: 9214605]
• Webber MM, Bello D, Kleinman HK et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis. 1996-08-01 [PMID: 8761420]
• Clancy AN, Whitman C, Michael RP et al. Distribution of androgen receptor-like immunoreactivity in the brains of intact and castrated male hamsters. Brain Res Bull. 1994-01-01 [PMID: 8293317]
• Takeda H, Chodak G, Mutchnik S et al. Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor. J Endocrinol. 1990-07-01 [PMID: 2199591]
• Clancy AN, Bonsall RW, Michael RP et al. Immunohistochemical labeling of androgen receptors in the brain of rat and monkey. Life Sci. 1992-01-01 [PMID: 1734159]

Bioinformatics

• Gene Symbol: AR
• Entrez:
  • 367(Human)
  • 11835(Mouse)
  • 24208(Rat)
• Uniprot:
  • P10275()