Androgen R/NR3C4 Antibody (AR 441)

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (AR 441) [NB120-9474] - Analysis of Human Prostate.

Product Discontinued

Androgen R/NR3C4 Antibody (AR 441) Summary

• Immunogen: BALB/C mice were injected with a synthetic peptide corresponding to the human androgen receptor.
• Localization: Nuclear
• Specificity: This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length AR and also with the newly described A form of the receptor. This does not cross react with estrogen, progesterone or glucocorticoid receptors.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AR
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
• Application Notes: For IHC: formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.
• Control:

  Human Prostate Whole Tissue Lysate (Adult Whole Tumor)

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Citrate, pH 6.0, containing less than 0.1% sodium azide
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Androgen R/NR3C4 Antibody (AR 441)

• AIS
• Androgen R
• androgen receptor
• AndrogenR
• AR
• DHTR
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• NR3C4
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. Androgen receptor status may be a useful marker in assessing triple-negative breast cancer. The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Androgen R/NR3C4 Antibody (NB120-9474)(1)

Publication using NB120-9474

• Thadani-Mulero, M, Portella, L Et al. Androgen receptor splice variants determine taxane sensitivity in prostate cancer. Cancer Res 2014-04-15 [PMID: 24556717] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: AR
• Entrez:
  • 367(Human)
• Uniprot:
  • P10275()