AMPK beta 2/PRKAB2 Antibody (1092615) [Unconjugated]

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human AMPK beta 2 Monoclonal Antibody (Catalog # MAB11639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta 2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta 2 at approximately 38 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK beta 2 Monoclonal Antibody (Catalog # MAB11639). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 1092615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

AMPK beta 2/PRKAB2 Antibody (1092615) [Unconjugated] Summary

• Immunogen: E. coli- derived recombinant human AMPKbeta2
  Met1-Ile272
  Accession # O43741
• Specificity: Detects recombinant human AMPK beta 2 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK beta 2/PRKAB2 Antibody (1092615) [Unconjugated]

• 5'-AMP-activated protein kinase, beta-2 subunit
• AMPK beta 2
• AMPK beta 2,5'-AMP-activated protein kinase subunit beta-2
• AMPK beta-2 chain
• AMPK subunit beta-2
• MGC61468
• PRKAB2
• protein kinase, AMP-activated, beta 2 non-catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2subunit in liver, while beta 2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O43741()