AMPK alpha 2 Antibody

Western Blot: AMPK alpha 2 Antibody [NBP1-52073] - Staining of Mouse (A) and Rat (B) Heart lysate

Western Blot: AMPK alpha 2 Antibody [NBP1-52073] - Western blot analysis of AMPK alpha 2 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-52073 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

AMPK alpha 2 Antibody Summary

• Immunogen: Peptide with sequence CPLDALNTTKP corresponding to internal region according to NP_006243.2.
• Epitope: CPLDALNTTKP
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRKAA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: EIA: Sandwich ELISA with increasing amount of recombinant PRKAA2 captured by a rabbit antibody. WB: Approx. 65 kDa band observed in human heart and liver lysates and in mouse and rat heart lysates (calculated MW of 62.3 kDa band according to NP_006243.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMPK alpha 2 Antibody

• 5'-AMP-activated protein kinase catalytic subunit alpha-2
• AMPK alpha 2
• AMPK subunit alpha-2
• AMPK2
• AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
• AMPK-alpha-2 chain
• EC 2.7.11
• EC 2.7.11.1
• PRKAA
• PRKAA2
• protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAA2
• Entrez:
  • 5563(Human)
  • 108079(Mouse)
  • 78975(Rat)
• Uniprot:
  • NP_006243.2(Human)