Aminopeptidase LRAP/ERAP2 Antibody (3F5)

• Reactivity: Hu
• Applications: WB
• Clone: 3F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Aminopeptidase LRAP/ERAP2 Antibody (3F5) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase LRAP/ERAP2
  Accession # Q6P179
• Specificity: Detects human Aminopeptidase LRAP/ERAP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human PILS is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERAP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase LRAP/ERAP2 Antibody (3F5)

• Aminopeptidase LRAP
• EC 3.4.11
• EC 3.4.11.3
• endoplasmic reticulum aminopeptidase 2
• ERAP2
• FLJ23633
• FLJ23701
• FLJ23807
• Leukocyte-derived arginine aminopeptidase
• LRAPL-RAPEC 3.4.11.-

Background

Leukocyte-derived arginine aminopeptidase (LRAP) and endoplasmic reticulum-aminopeptidase 2 (ERAP2) are the two names given to the protein encoded by the LRAP gene (1, 2). Induced by interferon-gamma , LRAP is able to trim various MHC class I antigenic peptide precursors. It belongs to the oxytocinase subfamily of M1 aminopeptidases, which also includes aminopeptidases PILS/ARTS1/ERAP1 and LNPEP/PLAP (1, 3). In addition to antigen presentation, the members of this subfamily are also important in maintenance of pregnancy, memory retention, and blood pressure regulation (3).

1. Tanioka, T. et al. (2003) J. Biol. Chem. 278:32275.
2. Tanioka, T. et al. (2005) FEBS J. 272:916.
3. Tsujimoto, M. and A. Hattori (2005) Biochim. Biophys. Acta 1751:9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase LRAP/ERAP2 Antibody (MAB3830)(5)

Publications using MAB3830

• L Giusti, M Tesi, F Ciregia, L Marselli, L Zallocco, M Suleiman, C De Luca, S Del Guerra, M Zuccarini, M Trerotola, DL Eizirik, M Cnop, MR Mazzoni, P Marchetti, A Lucacchini, M Ronci The Protective Action of Metformin against Pro-Inflammatory Cytokine-Induced Human Islet Cell Damage and the Mechanisms Involved Cells, 2022-08-08;11(15):. 2022-08-08 [PMID: 35954309] (Western Blot, Human)
• K Textoris-T, C Cammann, P Henklein, E Topfstedt, F Ebstein, S Henze, J Liepe, F Zhao, D Schadendor, B Dahlmann, W Uckert, A Paschen, M Mishto, U Seifert ER-aminopeptidase 1 determines the processing and presentation of an immunotherapy-relevant melanoma epitope Eur. J. Immunol., 2019-11-27;0(0):. 2019-11-27 [PMID: 31729751] (Western Blot, Human)
• IC Kuo, HK Kao, Y Huang, CI Wang, JS Yi, Y Liang, CT Liao, TC Yen, CC Wu, KP Chang Endoplasmic reticulum aminopeptidase 2 involvement in metastasis of oral cavity squamous cell carcinoma discovered by proteome profiling of primary cancer cells Oncotarget, 2017-06-27;8(37):61698-61708. 2017-06-27 [PMID: 28977897] (Western Blot, Human)
• P Guasp, E Barnea, MF González-E, A Jiménez-Re, JR Regueiro, A Admon, JA López de C The Beh�et's disease-associated variant of the aminopeptidase ERAP1 shapes a low affinity HLA-B*51 peptidome by differential subpeptidome processing J. Biol. Chem., 2017-04-26;0(0):. 2017-04-26 [PMID: 28446606] (Western Blot, Human)
• Keller M, Ebstein F, Burger E, Textoris-Taube K, Gorny X, Urban S, Zhao F, Dannenberg T, Sucker A, Keller C, Saveanu L, Kruger E, Rothkotter H, Dahlmann B, Henklein P, Voigt A, Kuckelkorn U, Paschen A, Kloetzel P, Seifert U The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition. Eur J Immunol, 2015-10-19;45(12):3257-68. 2015-10-19 [PMID: 26399368] (Western Blot, Human)

Review for Aminopeptidase LRAP/ERAP2 Antibody (MAB3830) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22918227
• Species: Human

Bioinformatics

• Gene Symbol: ERAP2
• Uniprot:
  • Q6P179()