AMICA/JAML Antibody (4E10)

Images

 
Flow Cytometry: AMICA/JAML Antibody (4E10) [NBP1-43309] - Staining of mouse JAML-transfected CHO cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse JAML Purified ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IP
Clone
4E10
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

AMICA/JAML Antibody (4E10) Summary

Immunogen
The immunogen for this antibody was JAML.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
AMICA1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This 4E10 antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for AMICA/JAML Antibody (4E10)

  • adhesion molecule, interacts with CXADR antigen 1
  • AMICA
  • AMICA1
  • CREA7-1
  • Gm638
  • JAML

Background

The 4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gammadelta T cell lines, and can provide co-stimulatory signals for cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43309AF405
Alexa Fluor 488 Labeled NBP1-43309AF488
Alexa Fluor 647 Labeled NBP1-43309AF647
Alexa Fluor 700 Labeled NBP1-43309AF700
Allophycocyanin Labeled NBP1-43309APC
DyLight 350 Labeled NBP1-43309UV
DyLight 405 Labeled NBP1-43309V
DyLight 488 Labeled NBP1-43309G
DyLight 680 Labeled NBP1-43309FR
DyLight 755 Labeled NBP1-43309IR
PE Labeled NBP1-43309PE
PerCP Labeled NBP1-43309PCP

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Bioinformatics

Gene Symbol AMICA1
Entrez