AMICA/JAML Antibody (4E10) - BSA Free

Flow Cytometry: AMICA/JAML Antibody (4E10) [NBP1-43309] - Staining of mouse JAML-transfected CHO cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse JAML Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC . Total viable cells were used for analysis.

• Reactivity: Mu
• Applications: Flow, IP
• Clone: 4E10
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

AMICA/JAML Antibody (4E10) - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to residues C(267) D G I L G D N F R P T Q(279) of mouse CARP.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: JAML
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
• Application Notes: This 4E10 antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for AMICA/JAML Antibody (4E10) - BSA Free

• adhesion molecule, interacts with CXADR antigen 1
• AMICA
• AMICA1
• CREA7-1
• Gm638
• JAML

Background

The 4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gammadelta T cell lines, and can provide co-stimulatory signals for cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMICA/JAML Antibody (NBP1-43309)(1)

Publication using NBP1-43309

• Enriquez AB, Sia JK, Dkhar HK Et al. Mycobacterium tuberculosis impedes CD40-dependent notch signaling to restrict Th(17) polarization during infection iScience 2022-05-19 [PMID: 35586066] (FLOW, Mouse)
  
  Details:
  Citation using the PE/Cy7 version of this antibody.

Bioinformatics

• Gene Symbol: JAML