AMICA/JAML Antibody (401901) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
401901
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

AMICA/JAML Antibody (401901) [Alexa Fluor® 700] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human AMICA/JAML
Leu20-Leu275
Accession # Q86YT9
Specificity
Detects human AMICA/JAML in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse AMICA is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AMICA1
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMICA/JAML Antibody (401901) [Alexa Fluor® 700]

  • adhesion molecule, interacts with CXADR antigen 1
  • AMICA
  • AMICA1
  • CREA7-1
  • Gm638
  • JAML

Background

AMICA (adhesion molecule, interacting with CXADR antigen 1), also known as JAML, is a 65 kDa, heavily glycosylated transmembrane protein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily (1). JAM family molecules contribute to intercellular connections within epithelial and endothelial cell layers, and mediate their interactions with various hemopoietic cells (1). The human AMICA cDNA encodes a 384 amino acid (aa) precursor that includes a 19 aa signal sequence, a 256 aa extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain (2). Alternative splicing may generate isoforms with N- and C-terminal deletions. In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif (3). Within the ECD, human AMICA shares 58% and 63% aa sequence identity with mouse and rat AMICA, respectively. It shares 18%‑20% aa sequence identity with the ECDs of human JAM-A, -B, -C, and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is upregulated during the differentiation of myeloid leukemia cells (2, 3). A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA (2). AMICA mediates the adhesion of monocytes to endothelial cells (2) and neutrophil migration across epithelial cell monolayers (3). This latter function involves specific interactions of AMICA with the coxsackie virus and adenovirus receptor (CXADR) in epithelial tight junctions (3). In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR (3). AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA (3).

  1. Mandell, K.J. and C.A. Parkos (2005) Adv. Drug Deliv. Rev. 57:857.
  2. Moog-Lutz, C. et al. (2003) Blood 102:3371.
  3. Zen, K. et al. (2005) Mol. Biol. Cell 16:2694.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB34491U
Alexa Fluor 405 FAB34491V
Alexa Fluor 488 FAB34491G
Alexa Fluor 594 FAB34491T
Alexa Fluor 647 FAB34491R
Alexa Fluor 700 FAB34491N
Alexa Fluor 750 FAB34491S
Allophycocyanin FAB34491A
Phycoerythrin FAB34491P

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Bioinformatics

Gene Symbol AMICA1