CRALBP Antibody (B2)

Images

 
Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.
Immunocytochemistry/ Immunofluorescence: CRALBP Antibody (B2) [NB100-74392] - Immunostaining of CRALBP in frozen mouse retina sections.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CRALBP Antibody (B2) Summary

Immunogen
Human recombinant CRALBP.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RLBP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.02 ug/ml
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
ELISA, IP, WB reported in the literature (PMID: 1715867) ICC reported in the literature (PMID:11909957)
Positive Control
CRALBP Lysate (NBL1-15383)
Publications
Read Publications using
NB100-74392 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CRALBP Antibody (B2)

  • Cellular retinaldehyde-binding protein
  • cellular retinaldehyde-binding protein-1
  • CRALBPretinaldehyde-binding protein 1
  • MGC3663
  • retinaldehyde binding protein 1

Background

The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRALBP Antibody (NB100-74392)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-74392 Applications Species
Biesecker Kr, Srienc Ai, Shimoda Am et al. Glial Cell Calcium Signaling Mediates Capillary Regulation of Blood Flow in the Retina. J. Neurosci. Sep 7 2016 [PMID: 27605617] (IHC-P) IHC-P
Heller JP, Kwok JC, Vecino E et al. A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases. Front Cell Neurosci 2015 [PMID: 26635529] (Rat) Rat
Unterlauft JD, Eichler W, Kuhne K et al. Pigment Epithelium-Derived Factor Released by Muller Glial Cells Exerts Neuroprotective Effects on Retinal Ganglion Cells Neurochem Res 2012 Mar 13 [PMID: 22410737] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Arnold TD, Ferrero GM, Qiu H et al. Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin [alpha]V[beta]8-Mediated Activation of Transforming Growth Factor-[beta]. J Neurosci 2532(4):1197-12. 2012 Jan. [PMID: 22279205]
Lu B, Malcuit C, Wang S et al. Long-term safety and function of RPE from human embryonic stem cells in preclinical models of macular degeneration. Stem Cells 2009 Sep [PMID: 19521979] (WB, Human) WB Human
Budhu AS, Noy N. Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest. Mol Cell Biol. 2002 Apr. [PMID: 11909957]
Crabb JW, Gaur VP, Garwin GG et al. Topological and epitope mapping of the cellular retinaldehyde-binding protein from retina. J Biol Chem. 1991 Sep. [PMID: 1715867]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRALBP Antibody (NB100-74392) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RLBP1
Entrez
Uniprot