alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of various lysates, using AMACR antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 7X5Z2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human alpha-Methylacyl-CoA Racemase/AMACR (NP_055139.4). MALQGISVVELSGLAPGPFCAMVLADFGARVVRVDRPGSRYDVSRLGRGKRSLVLDLKQPRGAAVLRRLCKRSDVLLEPFRRGVMEKLQLGPEILQRENPRLIYARLSGFGQSGSFCRLAGHDINYLALSGVLSKIGR
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AMACR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

• 2-methylacyl-CoA racemase
• alpha-Methylacyl-CoA Racemase
• alphaMethylacylCoARacemase
• AMACR
• AMACRD
• CBAS4
• EC 5.1.99.4
• RACE
• RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMACR