alpha-Galactosidase A/GLA Overexpression Lysate (Native)

Images

 
Western Blot: Galactosidase alpha Overexpression Lysate (Adult Normal) [NBL1-11104] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Galactosidase alpha.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

alpha-Galactosidase A/GLA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201304 and based on accession number NM_000169. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens galactosidase, alpha (GLA), mRNA.
Gene
GLA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for alpha-Galactosidase A/GLA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for alpha-Galactosidase A/GLA Overexpression Lysate (Native)

  • agalsidase alfa
  • Agalsidase alpha
  • Agalsidase
  • Alpha-D-galactosidase A
  • alpha-D-galactoside galactohydrolase 1
  • Alpha-D-galactoside galactohydrolase
  • alpha-gal A
  • alpha-galactosidase A
  • EC 3.2.1
  • EC 3.2.1.22
  • GALA
  • galactosidase, alpha
  • GLA
  • Melibiase

Background

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for alpha-Galactosidase A/GLA Lysate (NBL1-11104) (0)

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Video Protocols

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Bioinformatics

Gene Symbol GLA
Entrez