alpha-Galactosidase A/GLA Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC201304 and based on accession number NM_000169. The protein contains a C-terminal DDK tag.
Homo sapiens galactosidase, alpha (GLA), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for alpha-Galactosidase A/GLA
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for alpha-Galactosidase A/GLA Overexpression Lysate (Native)
- agalsidase alfa
- Agalsidase alpha
- Alpha-D-galactosidase A
- alpha-D-galactoside galactohydrolase 1
- Alpha-D-galactoside galactohydrolase
- alpha-gal A
- alpha-galactosidase A
- EC 3.2.1
- EC 22.214.171.124
- galactosidase, alpha
This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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