alpha-Galactosidase A/GLA Overexpression Lysate (Native)


Western Blot: Galactosidase alpha Overexpression Lysate (Adult Normal) [NBL1-11104] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Galactosidase alpha.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

alpha-Galactosidase A/GLA Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201304 and based on accession number NM_000169. The protein contains a C-terminal DDK tag.
Homo sapiens galactosidase, alpha (GLA), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for alpha-Galactosidase A/GLA

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for alpha-Galactosidase A/GLA Overexpression Lysate (Native)

  • agalsidase alfa
  • Agalsidase alpha
  • Agalsidase
  • Alpha-D-galactosidase A
  • alpha-D-galactoside galactohydrolase 1
  • Alpha-D-galactoside galactohydrolase
  • alpha-gal A
  • alpha-galactosidase A
  • EC 3.2.1
  • EC
  • GALA
  • galactosidase, alpha
  • GLA
  • Melibiase


This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Hu
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Species: Hu
Applications: WB, ICC/IF, IP
Species: Hu, Po, Ca, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Species: Hu
Applications: WB, IHC, IHC-P

Publications for alpha-Galactosidase A/GLA Lysate (NBL1-11104) (0)

There are no publications for alpha-Galactosidase A/GLA Lysate (NBL1-11104).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for alpha-Galactosidase A/GLA Lysate (NBL1-11104) (0)

There are no reviews for alpha-Galactosidase A/GLA Lysate (NBL1-11104). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for alpha-Galactosidase A/GLA Lysate (NBL1-11104) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional alpha-Galactosidase A/GLA Products

Bioinformatics Tool for alpha-Galactosidase A/GLA Lysate (NBL1-11104)

Discover related pathways, diseases and genes to alpha-Galactosidase A/GLA Lysate (NBL1-11104). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha-Galactosidase A/GLA Lysate (NBL1-11104)

Discover more about diseases related to alpha-Galactosidase A/GLA Lysate (NBL1-11104).

Pathways for alpha-Galactosidase A/GLA Lysate (NBL1-11104)

View related products by pathway.

PTMs for alpha-Galactosidase A/GLA Lysate (NBL1-11104)

Learn more about PTMs related to alpha-Galactosidase A/GLA Lysate (NBL1-11104).

Blogs on alpha-Galactosidase A/GLA

There are no specific blogs for alpha-Galactosidase A/GLA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

BMP-2 [Unconjugated]

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our alpha-Galactosidase A/GLA Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol GLA