alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free

• Reactivity: Hu
• Applications: Simple Western, ELISA
• Clone: 4A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free Summary

• Description: Novus Biologicals Mouse alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free (NB110-7961) is a monoclonal antibody validated for use in Simple Western. Anti-alpha-Fetoprotein/AFP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human alpha-fetoprotein.
• Localization: Secreted
• Specificity: No cross-reaction with human albumin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 1:100-1:2000
  • Simple Western
• Application Notes: We recommend the following pair in sandwich immunoassay (capture-detection): NB110-7961 - NB120-10072. See Simple Western Antibody Database for Simple Western validation: separated by size; matrix was 12-230 kDa; detected by Chemiluminescence.
• Control:

  alpha-Fetoprotein/AFP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Fetoprotein/AFP Antibody (NB110-7961)(1)

Publication using NB110-7961

• Yin J, Niu J, Huo J et al. Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique Nutrients 2023-03-21 [PMID: 36986252]

Bioinformatics

• Gene Symbol: AFP
• Entrez:
  • 174(Human)
• Uniprot:
  • P02771(Human)