alpha-Actinin 1 Antibody (CB11)

Product Discontinued

alpha-Actinin 1 Antibody (CB11) Summary

• Immunogen: The antibody reacts with the alpha-Actinin antigen.
• Marker: Z-disk Marker
• Specificity: The antibody is specific to the 100 kD a-actinin polypeptide and reacts with human and chicken cells. Monoclonal antibody to a-actinin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified a-actinin from chicken gizzard was used as an immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Immunohistochemical stainings on frozen sections, western blotting and other immunoassays for cellular and tumor biology.Working dilution in western blotting is at least 1:1000.

Reactivity Notes

May react with Chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 1% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for alpha-Actinin 1 Antibody (CB11)

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN1